济南星宝生物技术有限公司

产品大类

天博体育官网app登陆信息

购物指南

首页→产品分类目录→分子生物→蛋白相关→1ml His GraviTrap Kit

1ml His GraviTrap Kit

GalaxyBio Code

包装规格

价格

说明书

购物车

DC120

10tests

1600元

1ml His GraviTrap Kit

————适合重力分离预装柱His-tagged重组蛋白，

货号	产品名称	规格	价格元
Cat：DC120	1ml Ni-NTA Agarose	10支	1600

■包装内容

His GraviTrap 层析柱（预装1 ml Ni-NTA Agarose）

10支

Ni-NTA Agarose 10ml

Guanidine HCl 50g

Urea 100g

1 M Imidazole, pH 8.0 50ml

5x Phosphate Buffer Stock Solution

(0.5 M NaH2PO4; pH 8.0) 100 ml

Control Vector DNA 1 μg

■产品特点

Column material	Polypropylene barrel, polyethylene frits
Medium	Ni Sepharose 6 Fast Flow
Average bead size	90 μm
Protein binding capacity	Approx. 40 mg histidine-tagged protein/column
Bed volume	1 ml
Compatibility during use	Stable in all commonly used buffers, reducing agents, denaturants and detergents. (See Table 2.)
Avoid in buffers	Chelating agents, e.g. EDTA, EGTA, citrate.
pH stability,short-term(2 h)	2–14
Storage	20% ethanol
Storage temperature	+4 to +30 ºC

■ 产品说明：

含10根装有1ml Ni Sepharose 6 FF凝胶的His Gravitrap预装柱和His Buffer kit；每注可处理10ml样品和吸附40mg His标记蛋白质；

蛋白纯化流程如下：